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Case 41 - Discussion

Uploaded: 2008-01-18, Updated: 2008-01-18

 

 

IMMUNOHISTOCHEMISTRY STAINS

   

CD3

CD5

CD20

CD79a

LCA

PAX5

CK AE1/3

CD23

Pancytokeratin cocktail

CK 8/18

CD34

Vimentin

 

 

The tumor cells show strong positivity for vimentin and focal positivity for CK 8/18, and focally weak positivity for CD45 andCD79a. They are negative for S100, synaptophysin, chromogranin, AE1/AE3 (pan CK), pancytokeratin cocktail, CD20, CD3, CD5, CD10, BCL6, CD23, PAX5, CD43, TdT, and CD34.

 

FLOW CYTOMETRY INTERPRETATION

 

INTERPRETATION:  An aberrant population of cells expressing CD45, CD138, CD8 and monotypic light chain kappa is identified at 22% of the total events analyzed.  The immunophenotype of those aberrant cells are similar to the abnormalcells in the bone marrow.

 

 Total events           A Bright CD45 positive 
 analyzed = 49240 	events = 1167 (22% of total
  CD45+                          100%
  CD19+/CD5-           		  <2%
  CD5+/CD19-                      15%
  CD5+/CD19+                      <2%
  Total CD3+                      10%
  CD3+/CD4+          		   4%
  Total CD8+			  70%
  CD3+/CD8+        		   6%
  Total CD138+			  45%
  sIg Kappa+			  41%
  sIg Lambda+			  <2%
            	Gated on the CD19+ events
  CD19+/sIg Kappa+             CD19<2%
  CD19+/sIg Lambda             CD19<2%

 

MOLECULAR STUDY

 

Molecular tests for IgH Gene Rearrangement were performed on the specimen from GE junction.

The test result is reported as: Positive for Monoclonal IgH Gene Rearrangement.

 

CASE INTERPRETATION AND DIAGNOSIS

  • Plasmablatic Lymphoma