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ELECTRON MICROSCOPY
Ultrastructurally, the neoplasm consists of sheets of neoplastic
spindle-shaped cells embedded in a collagen-like matrix. The
neoplastic cells contain long, spindly, irregular nuclei with
chromatin clumping and occasional prominent nucleoli. The neoplastic
cell cytoplasm contains abundant rough endoplasmic reticulum with
occasionally dilated cisterna. Some of the cells have intermediate
filaments with dense bodies approximating the plasma membrane.
Pinocytic vacuoles are inconspicuous. A very occasional lipid
vacuole is noted within these cells. Intermixed amongst the
neoplastic cells are macrophages and occasional capillary. No
mitotic figures are noted. ULTRASTRUCTURAL IMPRESSION: Spindle cell
neoplasm.
CYTOGENETICS REPORT
NOMENCLATURE:
48,XX,dup(4)(p13p15.1),+18,+der(22)r(22;17;?)(p12q13;q21q25;?)[20]
Cytogenetic analysis revealed the presence of a hyperdiploid clone
characterized by a duplication of a portion of 4p, trisomy 18, and a
supernumerary ring chromosome comprised of 22q, 17q and unknown
material. These findings, specifically the presence of the ring
chromosome, support the clinical diagnosis of dermatofibrosarcoma
protuberans. |